Jasdeep Chatrath Padaria
National Research Centre on Plant Biotechnology, New Delhi
Title: Transcriptome Profiling for Identifying Genes to Develope Abiotic Stress Tolerant Transgenic Wheat
Biography
Biography: Jasdeep Chatrath Padaria
Abstract
Wheat (Triticum sp.) stands as the second largest staple crop of the world with 17% of the total cultivatable land under wheat production. Global annual wheat production needs to be increased at quantum leaps from the present production of more than 650 million metric tonnes so as to feed the ever burgeoning world population. Unfortunately, with the changing global climate, various abiotic stresses further hamper the wheat productivity. Development of abiotic stress tolerant cultivars is necessary to achieve the goal of enhanced wheat productivity. With the available gene pool within a species becoming limited, it becomes imperative that we search genes responsible for abiotic stress tolerance across the species, genus and even kingdom and using r- DNA technology develop transgenic wheat tolerant to abiotic stresses. The present study involved identification of abiotic stress responsive genes from tolerant plant systems as Penniseteum glaucum, Triticum aestivum , Ziziphus nummularia and Prosopis cineraria using Roche 454 and Illumina sequencing platforms. De novo assembly and transcriptome annotations were preformed to have insight about genes, gene family and transcriptional factors related to abiotic stress. Further analysis for change in expression level of known and unknown genes, SNP detection and SSR marker detection have been carried out to identify stress responsive genes and stress tolerance linked markers. A few stress responsive genes as NAC, P5CS, WRKY, HSP, MYB, ASR, DREB etc have been identified and characterized. These genes are further functionally validated and have been transformed in elite Indian bread wheat for development of transgenic wheat tolerant to abiotic stress.