Tetiana Ivchenko
Institute of Vegetables and Melon Growing NAAS, Ukraine
Title: The usage of cell selection in vitro for creation of tomato and eggplants breeding lines with resistance to Fuzarium oxysporum
Biography
Biography: Tetiana Ivchenko
Abstract
Development of a biotechnological system of assessment and selection of the forms of egg-plant and tomato those are tolerant to Fusarium oxysporum in the culture of the in vitro isolated tissues have been studied. It has been determined that samples can be reliably differentiated in the selective media with culture filtrates on the stages of induction and proliferation of callus according to their resistance in the field. The studies used the high-grade and hybrid seeds of egg-plant and tomato genotypes of F1 - F4 breeds with different tolerance to Fusarium oxysporum. Cell selection was carried out in the media with different content of selective agent (20, 40 and 60 % of the total medium's volume). According to the selective assessment of initial and selected through the cell selection new breeding lines of egg-plant and tomato, there were 4 promising lines of egg-plant and 5 lines of tomato highlighted that exceeded the control samples and the initial genotypes in their resistance to fusarium and in fertility. The possibility and efficiency of the biotechnology of accelerated creation of and express-tests on the breeding lines of egg-plant and tomato resistant to F. oxysporum were substantiated and experimentally proved in order to cut the time necessary for the attainment of resistant initial material.