Plant Genomics

Biography

Biography: Songli Yuan

Abstract

The root nodule symbiosis (RNS) between legume plants and rhizobia is the most efficient and productive source of nitrogen fixation, and has critical importance in agriculture and mesology. Soybean (Glycine max), one of the most important legume crops in the world, normally establishes a nitrogen-fixing symbiosis with different types of rhizobia, and the efficiency of symbiotic nitrogen fixation in soybean by application of inoculants greatly depends on the symbiotic host-specificity. Surface polysaccharides, secretion proteins of the type-three secretion systems and nod factors are used by rhizobia to modulate host range, but the host control of nodulation specificity remains poorly understood. To study whether there are some “mark genes” for the identification of soybean responding to different species rhizobium or not, we tested the matching abilities between several main planting soybean varieties and 10 various high nodulation Rhizobia strains (8 of which originating from different locations in China and 2 from America), and finally we took two symbiotic systems: Bradyrhizobium japonicum strain 113-2- Soybean Tian long No.1 (Oil Crops Research Institute CAAS in China, can form RNS with all of the ten Rhizobium) and Sinorhizobium fredii USDA205- Soybean Tian long No.1, which possess notable different nodulation phenotypes, as research subjects and investigated their differentially expressed genes (DEGs) in soybean roots at 5 different post-inoculation time points using RNA-seq. The DEGs uncovered in this study provides molecular candidates for better understanding the mechanisms of symbiotic host-specificity and explaining the different symbiotic effects between soybean roots inoculated with different strains (113-2 and USDA205).