Plant Genomics

Biography

Biography: Chaturvedi Rakhi

Abstract

      Tea       is  a      perennial     , e vergreen tree of the family Theaceae. It is most consumable non-alcoholic  caffeine containing      beverage      in the world due to  its pleasant taste, attractive aroma and its medicinal property. Tea contains large amount of      Catechins      (a group of very active     flavonoids    ) which have    anticancer   ,   antidiabetic  ,  antiviral ,  antimala   rial , hepatoprotective, ne uroprotective and c ardio  protective effects. Present study aims to study batch kinetics of   androgenic haploid cell   lines established from  pollen grains  in anther cultures of TV21 cultivar of tea. The haploid status  of these cell  lines were confirmed by flow cytometry. Cell suspension cultures were raised from fresh friable and high proliferating calli established on semi-solid medium. Dynamic changes of parameters, such as pH, fresh and dry cell concentrations, consumption of major nutrients, carbon source and agitation speeds, were studied to understand the culture characteristics. Maximum viable cultures were obtained at a rotation speed of 120 rpm. Identification and quantification of    Caffeine   , (+)-Catechine, (-)-Epicatechin and (-)-Epigallocatechin gallate were performed by HPLC which were further confirmed through Mass spectrometric data analysis. Maximum yield of 2.7%   Caffeine  , 0.26 % (+)- Catechin , 0.92% (-)-Epicatechin, and 10.36% (-)-Epig allocatechin gallate per gram dry weight was obtained from cells in suspension culture at 24th day. Compare to this, leaves from parent plant prod uced 3.14% Caffeine, 0.48 % (+)-Catechin, 1.3% (-)-Epicatechin, and 11.3% (-)-Epigallocatechin gallate per gram dry weight which was marginally higher. The optimized conditions and parameters of the pr esent study would be useful for further scale-up process by using suitable elicitors/precursors.