MD. MONIRUL ISLAM
ACI-RDA R&D Station, Bangladesh, India
Title: SCREENING OF SALT TOLERANT RICE MUTANTS /VARIETIES USING SSR MARKERS AT SEEDLING STAGE
Biography
Biography: MD. MONIRUL ISLAM
Abstract
Molecular markers especially, microsatellite or simple sequence repeats (SSRs) was the marker aided selection (MAS) technique utilized to determine salinity tolerance in rice. It accelerates rice breeding process by significantly improving selection efficiency. Fourty varieties were used to study the effect of salt stress on seedling growth and efficiency of SSR markers for the screening of salt tolerant varieties. Phenotyping of the varieties at the seedling stage was done using salinized (EC=12ds/m) culture solution under controlled conditions at the Bangladesh Institute of Nuclear Agriculture (BINA) glasshouse. Salt tolerant varieties showed stunted growth with the reduction of leaf area over the varieties of non-saline conditions. The seedlings grown from the normal seeds were vigorous than the seedlings from the seeds of saline conditions. This result indicated the impediment of seed vigor due to salt stress. The salt tolerant varieties from saline conditions were used to evaluate the efficiency of salt stress linked markers viz., RM231, RM24, RM334, RM32 and RM219 which were selected from the previous studies at IRRI in salinity screening program of known tolerant variety Pokkali and susceptible variety IR29. The efficiency was estimated by calculating the ratio of salt tolerant varieties (phenotypically) in saline conditions and amplification of markers against the same