Soumitra Paul
University of Calcutta,India
Title: Molecular analysis of bioengineered rice: A proteomic and transcriptomic approach
Biography
Biography: Soumitra Paul
Abstract
Food security is a major challenging issue in developing countries like India where rice is the staple crop . Abiotic stress like drought affe cts most of the rice production in India. The ge neration of drought tolerant rice cultiva r by overexpression AtDREB1A is an important breakthrough in biotechnology resear ch. In addition, rice contains very little amount of iron and zinc in the edible endosperm . Therefore, biofortification of these two micronutrients in rice grains i s believed to be the most promising strategy for enhancing mineral nutrition in diets. The bioengineered high iron rice grain has already been developed by overexpression of soybean and rice endogenou. Low phytate rice grain by s ferritin genes RNAi mediated silencing of phytic acid biosynthesis also facilitates iron accumulation in milled rice grain. However, the molecula r signaling network operating in transgenic crops still remains unclear. In our cu rrent study, we performed the over expressin proteomic analysis of DREB1A g drought tolerant rice cultivars. The proteome profile of rice roots revealed metabol ic alteration in energy and carbohydrate metabolism. A novel r40c1 protein species has been identified which play a cruc ial role in DREB1A mediated drought tolerance. Recently, the role of miRNAs in iron transport has been deciphered in transgenic ferritin over expressing rice plants which unravel the molecular mechanism of iron loading in high iron rice grains. Four novel miRNAs targeting NRAMP4 has been considered for enhancement of iron loading in transgenic rice plants.