Ananya prova
EXIM Bank Agricultural University, Bangladesh
Title: ISOLATION, IDENTIFICATION AND CHARACTIZATION OF WHITE MOLD DISEASE IN MARIGOLD (Tagetes officinales L.) AND HYACINTH BEAN (Lablab purpureus) CASUED BY Sclerotinia sclerotiorum
Biography
Biography: Ananya prova
Abstract
During January 2011, while surveying for different diseases, suspected symptoms of Sclerotinia stem and blossom rot were observed on African marigold in flower garden of Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh. Similarly, plant with light tan to brown blighted stems and pods were observed in the hyacinth bean field. White fluffy mycelium and production of cylindrical black sclerotia on and inside infected plant parts were also observed. Isolations from surface-disinfested flower petals and stems onto potato dextrose agar (PDA) consistently yielded white, fluffy colonies with a ring of sclerotia near the edge of PDA plates typical of Sclerotinia sclerotiorum (Lib.) de Bary. Pathogenecity tests were carried out for both isolates according to Koch’s postulate. The optimal temperature for mycelial growth ranged from 15 to 20°C. Optimum pH for radial growth was pH 5.0 to 6.0 in that four carbon sources except starch. Sucrose and Mannitol were found to be the best carbon sources media to support hyphal growth followed by glucose. As well as sucrose was the best medium for sclerotial development yielding highest sclerotial weight.In-vitro evaluation of different microbial antagonists, plant extracts and fungicides were reavealed that, Bavistin, Dithane M-45, Rovral, extract of eucalyptus, turmeric, and neem and Trichoderma harzianum were found to be most effective for significantly inhibition of colony growth of S. sclerotiorum at highest as well as at lowest concentration. Calcium (Ca) amendment in PDA media also significantly retarded the mycelial growth and sclerotial formation of S. sclerotiorum.