Naglaa A. Ashry
Agricultural Research Center, Field Crops Research Institute, Egypt
Title: Identification of stress responsive gene from Acacia nilotica subsp nilotica
Biography
Biography: Naglaa A. Ashry
Abstract
This study focused on the effective abiotic stress responsive element known as Dehydration responsive element binding factor (DREB) in Acacia nilotica subsp nilotica as a crop legumes wild relative representing a candidate of genetic resource for many important genes. DREB protein is a subfamily of AP2/ERF transcription factors, which control expression of many drought and salinity inducible genes and encode tolerance to environmental stresses in many plants. The objective of this study was to identify and molecular characterize one of the most common abiotic stress related genes (DREB genes) as a transcription factor for abiotic stress genes. A novel DNA fragment was amplified using specific primer for Arabidopsis thaliana DREB gene using extracted genomic A. nilotica DNA. The resulted amplicone was sequenced and submitted to NCBI with the accession number JX539903. Similarity searching was performed to identify the similar sequences vs. isolated fragment using BLASTN tool at the NCBI database. The results showed similarity between Acacia amplified fragment and Medicago truncatula, G. max and A. thaliana as model biological systems. Translation of the fragment into hypothetical protein showed similarities with Glycosyltransferase genes known to have a role in plant abiotic stress tolerance.