G. Balasubramani
ICAR-Central Institute for Cotton Research,India
Title: Expression analysis of cotton fiber strength associated genes using RILs mapping population
Biography
Biography: G. Balasubramani
Abstract
Cotton is natural and renewable fiber. Cotton lint is priced based on the fiber properties. Presently textile industry use high speed open end rotor spinning machine which demand high strength (>30g/tex) and length (>2.8cm) with moderate fineness (3.7- 4.2 mic) of cotton lint. Most of the Indian varieties fail to fulfil the requirements especially strength. The discovery of the cotton cellulose synthase (GhcesA) genes encoding potential catalytic subunits of cellulose synthase revealed the involvement in biosynthesis of microfibrils of cellulose. A number of genes associated with fiber length (GhcesA1, GhcesA3, GhcesA5, GhcesA6 and GhcesA9) and strength (GhcesA1, GhcesA2, GhcesA7, GhcesA8, Ghfla3 and Ghcobl4) were identified. With that information we have studied the relative gene expression with GhcesA1, GhcesA2, GhcesA7, Ghcobl4, Ghfla3 and GhMT1genes using RILs mapping population and the results showed that GhcesA1, GhcesA2, Ghfla3 and Ghcobl4 had strong association and higher expression during secondary wall synthesis, which reveals the high fiber strength. Over expression and down regulation constructs for three potential genes such as GhcesA1, GhcesA2, and Ghfla3 were generated in plant transformation vector (pCAMBIA2300) driven by fibre specific promoter and the gene cassette were confirmed by restriction analysis. All the above genes are cloned under 990bp of GhcesA1 promoter sequences. Thus all the four genes were cloned for validation of their role by over expression as well as down regulation in low and high fiber strength genotypes of Indian cotton through transgenic approach.