Brijesh Pandey
Amity University, India
Title: Antioxidant profiling vis-a-vis growth and thymoquinne profiling in Nigella sativa
Biography
Biography: Brijesh Pandey
Abstract
Nigella sativa Linn., commonly known as the black cumin seed is an annual herb, belongs to the family of Ranuculaceae. The existing germplasm was analyzed for its antioxidant property at different stages of plant growth. Spectrophotometric analysis of peroxidases with guaiacol as substrate exhibited increase upto seed forming stage (0.127±0.055). While with o-dinididene maximum activity was observed at flowering stage and minor decline thereafter. Among non-enzymatic antioxidant flavonoids and total phenolics was found maximum at flowering stage (5.066±1.301) (mg equivalent QE/gram of tissue) ±SD and (20.44±1.596) (mg equivalent GA /gram of tissue) ±SD respectively. Total carotenoid and total antioxidant was observed maximum at seed forming stage (1148.51±36.385) (µg/gram fresh weight) ±SD and (0.952±0.135) (mM equivalent ascorbic acid/g tissue) ±SD respectively. When correlation was established between total antioxidant and different enzymatic and non enzymatic antioxidants, it was found to be strongly positive. The germplasm has been tested for extraction of thymoquinone (TQ), a potent antioxidant and analysed by HPLC. The growth profile for above mentioned antioxidants and TQ seems interesting and deserves special attanetion.