Scientific Program

Conference Series LLC Ltd invites all the participants across the globe to attend 8th World Congress on Plant Genomics and Plant Science Osaka, Japan.

Day 1 :

Keynote Forum

Rachel Swee-Suak Ko

Professor, Agricultural Biotechnology Research Center, Taiwan

Keynote: Phalaenopsis aphrodite (moth orchid)- functional genomics and biotechnology

Time : 09:30 AM - 10:15 AM

Plant Genomics 2018 International Conference Keynote Speaker Rachel Swee-Suak Ko photo
Biography:

Rachel Swee-Suak Ko works for Academia Sinica Agricultural Biotechnology Research Center/ BCST, Taiwan R.O.C.  She got her Ph.D. in National Chung Hsing University in Taiwan. The main focus of her research is to increase double spiking rate and increase flower production in Phalaenopsis orchid by manipulating the best environmental factors of CO2, LED lighting, and temperature. She is interested to understanding and discovers the complex molecular mechanisms that control spiking phenomenon in Phalaenopsis orchid. The basic knowledge gain will facilitate orchid production

Abstract:

Phalaenopsis aphrodite (moth orchid) is one of the most important ornamental crops in the international orchid market. In nature, this epiphytic orchid grows on the surface of trees. Phal. aphrodite has thick, succulent leaves and performs typical Crassulacean acid metabolism (CAM) photosynthesis. CAM orchids evolved to maximize carbon gain and simultaneously minimize water loss by opening their stomates at night under arid conditions, therefore, attaining high water use efficiency. However, several constraints such as slow growth rate, high ploidy levels, and large and complex of genome size might hamper basic study of this orchid.
The availability of next-generation sequencing technologies has greatly advanced the possibility of examination of sequence information in non-model crops. There are two orchid databases, named OrchidBase 2.0 and Orchidstra 2.0 freely available to the public in Taiwan. Notably, Orchidstra 2.0 provides transcriptomic resources for 18 orchid species including gene expression patterns in various tissues. The genome sequence of Phal. equestris was published in 2015, and recently, in April 2018, Academia Sinica further published the genome sequence of Phal. aphrodite. These databases and platforms facilitate functional genomics study and might speed up molecular breeding progress. In addition, several recent molecular technological advances might accelerate basic research, such as virus-induced gene silencing (VIGS) that provides transient, rapid testing for functional validation and screening of functional genes in orchids, and transient protoplast assay technology that can facilitate molecular study. Moreover, we developed a heritable and efficient Agrobacterium- mediated transformation using protocorms that enables functional genomics studies and molecular breeding.  
Here, I will present two cases of functional genomics studies of Phal. aphrodite: chloroplast movement and multiple spiking. Plants are sessile; therefore, they have evolved mechanisms to regulate growth and development to cope with ever-changing environments. In general, this orchid is very sensitive to high light irradiation, which can lead to burn spots on the leaves. Phototropins are blue light receptors in plants that function in chloroplast movement, stomatal opening, and affect plant growth and development. We have characterized the function of two PHOTOTROPIN genes in chloroplast movement. For the second case, multiple spike induction and its mechanism in Phal. aphrodite will be discussed. 

Keynote Forum

Magdy Montasser

Associate Professor , University of Kuwait, Biological Science

Keynote: Viral Satellite RNA as a Biological Control Agent Against Viral Diseases

Time : 10:15 AM - 11:00 AM

Plant Genomics 2018 International Conference Keynote Speaker Magdy Montasser  photo
Biography:

Magdy Montasser has his expertise in Molecular Virology and Plant Pathology over the last 30 years of experience in research, evaluation, teaching and supervising graduate students towards M.Sc. He optained his Ph.D. degree from Rutgers University, New Jersey, USA in 1988, and Post Doctor at the University of Maryland. Currently working as an Associate Professor at the University of Kuwait, Biological Science

 

Abstract:

A naturally occurring satellite RNA possessed by the CMV strain was first characterized and then used as a biological control agent to protect tomato plants against the disease induced by another severe strain. Viability and infectivity of the virus in extracted nucleic acid inoculums was further proved using mechanical transmission method. Tomato plants were pre inoculated or “vaccinated” with the strain containing viral satellite RNA, and then challenge inoculated with the severe strain at different time intervals.  All plants challenged three weeks after vaccination showed nearly complete protection from subsequent infection by the severe strain.  This biological control technology of plant viruses was effective and could be successfully used as biological control agent at the molecular level.