Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 8th World Congress on Plant Genomics and Plant Science Osaka, Japan.

Day 2 :

Keynote Forum

Zhang Daoyuan

Associate Professor, Xinjiang institute of Ecology and Geography, China

Keynote: Effects of Deficit Irrigation on the Growth, Yield and Quality of Cotton Overexpressing ScALDH21

Time : 09:30 AM - 10:15 AM

OMICS International Plant Genomics 2018 International Conference Keynote Speaker Zhang Daoyuan photo
Biography:

The molecular, cellular and biochemical responses of plants to water-deficit stress is the central, and long-term, interest of Daoyuan’s research program. Her basic research utilizes the desiccation-tolerant moss Syntrichia carninervis and Bryum argentum as model system for studying post-transcriptional gene control, cellular repair mechanisms, and as a source of novel tolerance-associated genes.
 
 

 

Abstract:

Plants have evolved complex molecular, cellular and physiological mechanisms to respond to environmental stressors. Although genetic manipulation has not been largely successful, it represents an important method for improving water deficit tolerance in agricultural crops. Aldehyde dehydrogenases involved in cellular responses to oxidative/electrophilic stress protect against a variety of environmental stressors. In the past, we cloned and characterized moss-unique ALDH21 homologue from Syntrichia caninervis (ScALDH21), an extremely desiccation-tolerant moss found in deserts of Central Asia.  ScALDH21 gene plays important roles in plant responses to abiotic stresses, as overexpression of this gene in tobacco and cotton decreases the sensitivity of these plants to drought and salt stresses, suggesting that the ScALDH21 gene plays a critical role in abiotic stress responses. To test the possibility that transgenic ScALDH21 cotton may be suitable for cultivating under water deficit conditions, the phenotype, physiological response and yield of transgenic ScALDH21 cotton were measured in drought pool and field experiments. We report here that overexpression of ScALDH21 in cotton results in higher net photosynthesis, less cell damage, more cell protective substances and better growth than wild-type cotton under drought stress in drought pool. As compared with none transgenic control, yield of transgenic cotton lines under reduced irrigation condition increased more than yield under normal irrigation conditions. Seed cotton yield in the field increased approximately 10.0% under normal irrigation conditions and increased approximately 21.0% under deficit irrigation conditions compared with control. Furthermore, fiber quality character in transgenic cotton lines increased than that of control under normal and deficit irrigation conditions. These results suggest that transgenic ScALDH21 cotton is a viable candidate material to improve crop yields in water-limited agricultural production systems.

 

Keynote Forum

Rachel Swee-Suak Ko

Agricultural Biotechnology Research Center, Taiwan

Keynote: Phototropins control chloroplast movement in Phalaenopsis aphrodite

Time : 10:15 AM - 11:00 AM

OMICS International Plant Genomics 2018 International Conference Keynote Speaker Rachel Swee-Suak Ko photo
Biography:

Rachel Swee-Suak Ko is currently working in Academia Sinica Agricultural Biotechnology Research Center/BCST, Taiwan, China. She has obtained her PhD in National Chung Hsing University in Taiwan. The main focus of her research is to increase double spiking rate and increase flower production in Phalaenopsis orchid by manipulating the best environmental factors of CO2, LED lighting and temperature. She is interested in understanding and discovers the complex molecular mechanisms that control spiking phenomenon in Phalaenopsis orchid.

Abstract:

Phalaenopsis aphrodite (moth orchid) is one of the most important ornamental crops in international trade. In nature, this epiphytic orchid grows on the surface of trees. It utilizes crassulacean acid metabolism photosynthesis that takes up CO2 at night. High light environment might cause photodamage in orchid. Plants are sessile; therefore, they have evolved mechanisms to regulate growth and developed to cope with ever-changing environments. Phototropins are blue light receptors in plants that function in chloroplast movement, stomatal opening and affect plant growth and development. Full-length cDNAs of two PHOT genes, PaPHOT1 and PaPHOT2 were cloned from Phalaenopsis aphrodite and their functions in chloroplast movement were investigated in this study. Phalaenopsis orchid responds to Blue Light (BL). Slit assay indicated that chloroplasts did not move at 20 μmole m-2s-1; however, in low BL (<15 μmole m-2s-1) orchids accumulated more chloroplasts in the periclinal cell walls. Chloroplasts started moving at BL>25 μmole m-2s-1. Significant chloroplast avoidance movement was observed at BL>100 μmole m-2s-1. Orchids consistently expressed higher levels of PaPHOT1 and PaPHOT2 under low BL. while, PHOT2 was up-regulated under high BL regimes. To verify the biological function of phototropins in chloroplast movement, Virus-Induced Gene Silencing (VIGS) was used. VIGS-treated orchid leaves showed decreasing gene expression of PHOTs and reduced chloroplast movement phenomena under high BL. In addition, we showed heterologously overexpressing PaPHOT1 and PaPHOT2 in Arabidopsis mutants recovered chloroplast movement. In conclusion, we successfully isolated and characterized two PHOTs from P. aphrodite and showed that they function in blue light-induced chloroplast movement.